RNA Biology

E-mail: walter.rossmanith(at)meduniwien.ac.at
Phone: 0043 1 40160 37512
Research Group: www.meduniwien.ac.at/wrlab/
Institution: MedUniWien

Full member of the DoktoratsKolleg (DK) RNA Biology since 2014.

PhD students within/associated to the DK:
Sabrina Summer
Enxhi Hazisllari

For complete list of group members please visit the Rossmanith group website.

tRNA biology & mitochondria

We are interested in the biogenesis of tRNA molecules, and the structure, function, and evolution of the enzymes involved in this multistep process. Though we now use and study various eukaryal model systems, the origin and focus of our research is the human mitochondrial system. In this small genetic subsystem, the tRNAs have non-canonical structures and are frequently involved in the pathogenesis of a heterogeneous group of diseases. We are interested in structure-function relationships of mitochondrial tRNAs, their biogenesis, and their role in disease. We employ a variety of experimental approaches and model systems for our studies.

 

Publications: (since 2014)

Deciphering the “m⁶A Code” via Antibody-Independent Quantitative Profiling.
Garcia-Campos MA, Edelheit S, Toth U, Safra M, Shachar R, Viukov S, Winkler R, Nir R, Lasman L, Brandis A, Hanna JH, Rossmanith W, Schwartz S.
Cell. 2019 Jul 25;178(3):731-747.e16.

A subcomplex of human mitochondrial RNase P is a bifunctional methyltransferase – extensive moonlighting in mitochondrial tRNA biogenesis.
Vilardo E, Nachbagauer C, Buzet A, Taschner A, Holzmann J, Rossmanith W.
Nucleic Acids Res. 2018 Nov 16;46(20):11126-11127.

Positioning Europe for the EPITRANSCRIPTOMICS challenge.
Jantsch MF, Quattrone A, O’Connell M, Helm M, Frye M, Macias-Gonzales M, Ohman M, Ameres S, Willems L, Fuks F, Oulas A, Vanacova S, Nielsen H, Bousquet-Antonelli C, Motorin Y, Roignant JY, Balatsos N, Dinnyes A, Baranov P, Kelly V, Lamm A, Rechavi G, Pelizzola M, Liepins J, Holodnuka Kholodnyuk I, Zammit V, Ayers D, Drablos F, Dahl JA, Bujnicki J, Jeronimo C, Almeida R, Neagu M, Costache M, Bankovic J, Banovic B, Kyselovic J, Valor LM, Selbert S, Pir P, Demircan T, Cowling V, Schäfer M, Rossmanith W, Lafontaine D, David A, Carre C, Lyko F, Schaffrath R, Schwartz S, Verdel A, Klungland A, Purta E, Timotijevic G, Cardona F, Davalos A, Ballana E, O Carroll D, Ule J, Fray R.
RNA Biol. 2018 May 9:1-3.

Minimal and RNA-free RNase P in Aquifex aeolicus.
Nickel AI, Wäber NB, Gößringer M, Lechner M, Linne U, Toth U, Rossmanith W, Hartmann RK.
Proc Natl Acad Sci U S A. 2017 Oct 17;114(42):11121-11126.

The m1A landscape on cytosolic and mitochondrial mRNA at single-base resolution.
Safra M, Sas-Chen A, Nir R, Winkler R, Nachshon A, Bar-Yaacov D, Erlacher M, Rossmanith W, Stern-Ginossar N, Schwartz S.
Nature. 2017 Nov 9;551(7679):251-255.

Protein-only RNase P function in Escherichia coli: viability, processing defects and differences between PRORP isoenzymes.
Gößringer M, Lechner M, Brillante N, Weber C, Rossmanith W, Hartmann RK.
Nucleic Acids Res. 2017 Jul 7;45(12):7441-7454.

Repairing tRNA termini: News from the 3′ end.
Rammelt C, Rossmanith W.
RNA Biol. 2016 Dec;13(12):1182-1188.

Analysis of the Cleavage Mechanism by Protein-Only RNase P Using Precursor tRNA Substrates with Modifications at the Cleavage Site.
Walczyk D, Gößringer M, Rossmanith W, Zatsepin TS, Oretskaya TS, Hartmann RK.
J Mol Biol. 2016 Dec 4;428(24 Pt B):4917-4928.

A novel HSD17B10 mutation impairing the activities of the mitochondrial RNase P complex causes X-linked intractable epilepsy and neurodevelopmental regression.
Falk MJ, Gai X, Shigematsu M, Vilardo E, Takase R, McCormick E, Christian T, Place E, Pierce EA, Consugar M, Gamper HB, Rossmanith W, Hou YM.
RNA Biol. 2016 May 3;13(5):477-85.

Substrate recognition and cleavage-site selection by a single-subunit protein-only RNase P.
Brillante N, Gößringer M, Lindenhofer D, Toth U, Rossmanith W, Hartmann RK.
Nucleic Acids Res. 2016 Mar 18;44(5):2323-36.

Mitochondrial poly(A) polymerase is involved in tRNA repair.
Fiedler M, Rossmanith W, Wahle E, Rammelt C.
Nucleic Acids Res. 2015 Nov 16;43(20):9937-49.

Distribution of Ribonucleoprotein and Protein-Only RNase P in Eukarya.
Lechner M, Rossmanith W, Hartmann RK, Thölken C, Gutmann B, Giegé P, Gobert A.
Mol Biol Evol. 2015 Dec;32(12):3186-93.

Molecular insights into HSD10 disease: impact of SDR5C1 mutations on the human mitochondrial RNase P complex.
Vilardo E, Rossmanith W.
Nucleic Acids Res. 2015 May 26;43(10):5112-9.

Playing RNase P evolution: swapping the RNA catalyst for a protein reveals functional uniformity of highly divergent enzyme forms.
Weber C, Hartig A, Hartmann RK, Rossmanith W.
PLoS Genet. 2014 Aug 7;10(8):e1004506.